Extended Abstract--Summary of Research
Shift of Acetaminophen Dosages on Liver,
Kidney, and Nerve Cells
The purpose of this research was to observe
the effect of off label dosages of Acetaminophen on Liver, Kidney, and Nerve
cells. Three separate hypotheses
were used. The main hypothesis
was that Acetaminophen applied at irregular dosage amounts would have a
noticeable effect on the cells. The null hypothesis, for statistical testing,
stated that irregular doses of Acetaminophen will have no effect on the
cells. Treatments of Acetaminophen
were calculated in to four treatment groups: control, low (0.05mg/mL), medium
(0.058 mg/mL), and high (0.58 mg/mL).
On Day 1 the cells were placed in the well plates.
On Day 2 treatments were sterilized and then added before overnight
incubation. On Day 3 the trypsin and trypan blue were added to the cells to
prepare them for counting. Using a
hemacytometer, the viability of the cells were counted.
For data analysis, ANOVA tests were ran. The data showed that increasing
dosages of Acetaminophen killed the liver cells the most (F= 7.4
, df= 15 , P=0.005
). This was somewhat
expected because Acetaminophen is processed in the liver.
Differences observed in the kidney and nerve cells were not statistically
significant. The kidney cells were
not affected whatsoever, and had viable cells in the thousands.
It was concluded that acetaminophen does have an effect on nerve and
liver cells. Further research is needed to determine if efforts need to be made
to reduce irregular acetaminophen
dosing.
Kidney, and Nerve Cells
The purpose of this research was to observe
the effect of off label dosages of Acetaminophen on Liver, Kidney, and Nerve
cells. Three separate hypotheses
were used. The main hypothesis
was that Acetaminophen applied at irregular dosage amounts would have a
noticeable effect on the cells. The null hypothesis, for statistical testing,
stated that irregular doses of Acetaminophen will have no effect on the
cells. Treatments of Acetaminophen
were calculated in to four treatment groups: control, low (0.05mg/mL), medium
(0.058 mg/mL), and high (0.58 mg/mL).
On Day 1 the cells were placed in the well plates.
On Day 2 treatments were sterilized and then added before overnight
incubation. On Day 3 the trypsin and trypan blue were added to the cells to
prepare them for counting. Using a
hemacytometer, the viability of the cells were counted.
For data analysis, ANOVA tests were ran. The data showed that increasing
dosages of Acetaminophen killed the liver cells the most (F= 7.4
, df= 15 , P=0.005
). This was somewhat
expected because Acetaminophen is processed in the liver.
Differences observed in the kidney and nerve cells were not statistically
significant. The kidney cells were
not affected whatsoever, and had viable cells in the thousands.
It was concluded that acetaminophen does have an effect on nerve and
liver cells. Further research is needed to determine if efforts need to be made
to reduce irregular acetaminophen
dosing.